A simple "universal" DNA extraction procedure using SDS and proteinase K is compatible with direct PCR amplification.
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چکیده
منابع مشابه
A simple "universal" DNA extraction procedure using SDS and proteinase K is compatible with direct PCR amplification.
Department of Medical Microbiology, University of Z/irich, 8028 Z/irich, Switzerland A large number of different protocols for the efficient isolation of highly purified DNA from eukaryotic and prokaryotic cells is extant. (1-4) These procedures usually include treatment with proteinase K in the presence of SDS, which efficiently lyses the cells and nuclei and liberates the DNA tightly bound in...
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ژورنال
عنوان ژورنال: Genome Research
سال: 1995
ISSN: 1088-9051
DOI: 10.1101/gr.4.6.368